optimal filter sets Search Results


dapi/cy5 (custom 239415) dual emission filters  (IDEX)
90
IDEX dapi/cy5 (custom 239415) dual emission filters
An overview of the reagents used for immunohistochemistry
Dapi/Cy5 (Custom 239415) Dual Emission Filters, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optimal+filter+sets/pmc10957212-147-15-18?v=IDEX
Average 90 stars, based on 1 article reviews
dapi/cy5 (custom 239415) dual emission filters - by Bioz Stars, 2026-08
90/100 stars
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filter sets optimized fluorophore  (IDEX)
90
IDEX filter sets optimized fluorophore
A. fluorescent proteins, without fused sequences, were continuously irradiated for 300 seconds with appropriate filter sets, with images captured every two seconds in live NIH 3T3 cells. Monochrome images were quantified using Image J (NIH), normalized to start fluorescent levels, and plotted over time. Deviations across three experiments for each <t>fluorophore</t> was <5%. B. PSCFP2 does not photoactivate with 175W unfiltered xenon light over 15 minutes constant irradiation. Light was provided without filtration through a liquid light guide and using a plan apochromat NA1.3 63X oil immersion objective (Nikon). While slight photobleaching was noted, (panels a versus c), no cyan fluorescence was detected above CCD camera noise (panels b,d). Scale bar is 10 μm.
Filter Sets Optimized Fluorophore, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optimal+filter+sets/pmc01523422-31-23-25?v=IDEX
Average 90 stars, based on 1 article reviews
filter sets optimized fluorophore - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
fitc/pe/apc optimized filter sets  (IDEX)
90
IDEX fitc/pe/apc optimized filter sets
A. fluorescent proteins, without fused sequences, were continuously irradiated for 300 seconds with appropriate filter sets, with images captured every two seconds in live NIH 3T3 cells. Monochrome images were quantified using Image J (NIH), normalized to start fluorescent levels, and plotted over time. Deviations across three experiments for each <t>fluorophore</t> was <5%. B. PSCFP2 does not photoactivate with 175W unfiltered xenon light over 15 minutes constant irradiation. Light was provided without filtration through a liquid light guide and using a plan apochromat NA1.3 63X oil immersion objective (Nikon). While slight photobleaching was noted, (panels a versus c), no cyan fluorescence was detected above CCD camera noise (panels b,d). Scale bar is 10 μm.
Fitc/Pe/Apc Optimized Filter Sets, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optimal+filter+sets/pmc08491950-400-25-29?v=IDEX
Average 90 stars, based on 1 article reviews
fitc/pe/apc optimized filter sets - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


An overview of the reagents used for immunohistochemistry

Journal: The Journal of Neuroscience

Article Title: Cholinergic Control of GnRH Neuron Physiology and Luteinizing Hormone Secretion in Male Mice: Involvement of ACh/GABA Cotransmission

doi: 10.1523/JNEUROSCI.1780-23.2024

Figure Lengend Snippet: An overview of the reagents used for immunohistochemistry

Article Snippet: At the same time, to detect Cy5 emission, a He–Ne laser (637 nm) and a DAPI/Cy5 (custom 239415; Semrock) dual emission filters were applied.

Techniques: Immuno-Electron Microscopy, Electron Microscopy

A. fluorescent proteins, without fused sequences, were continuously irradiated for 300 seconds with appropriate filter sets, with images captured every two seconds in live NIH 3T3 cells. Monochrome images were quantified using Image J (NIH), normalized to start fluorescent levels, and plotted over time. Deviations across three experiments for each fluorophore was <5%. B. PSCFP2 does not photoactivate with 175W unfiltered xenon light over 15 minutes constant irradiation. Light was provided without filtration through a liquid light guide and using a plan apochromat NA1.3 63X oil immersion objective (Nikon). While slight photobleaching was noted, (panels a versus c), no cyan fluorescence was detected above CCD camera noise (panels b,d). Scale bar is 10 μm.

Journal: Biological Procedures Online

Article Title: Practical three color live cell imaging by widefield microscopy

doi: 10.1251/bpo119

Figure Lengend Snippet: A. fluorescent proteins, without fused sequences, were continuously irradiated for 300 seconds with appropriate filter sets, with images captured every two seconds in live NIH 3T3 cells. Monochrome images were quantified using Image J (NIH), normalized to start fluorescent levels, and plotted over time. Deviations across three experiments for each fluorophore was <5%. B. PSCFP2 does not photoactivate with 175W unfiltered xenon light over 15 minutes constant irradiation. Light was provided without filtration through a liquid light guide and using a plan apochromat NA1.3 63X oil immersion objective (Nikon). While slight photobleaching was noted, (panels a versus c), no cyan fluorescence was detected above CCD camera noise (panels b,d). Scale bar is 10 μm.

Article Snippet: NIH 3T3 cells expressing 500ng of transiently transfected fluorophore-producing plasmids were imaged at 24 hours post transfection using filter sets optimized for each fluorophore (all Semrock sets).

Techniques: Irradiation, Filtration, Fluorescence